Founds et al. performed global gene expression profiling in first trimester placentas of women who later manifested preeclampsia to better seek clues to etiology and early circulating biomarkers of placental origin. Surplus chorionic villus sampling (CVS) tissues were collected at 10-12 weeks gestation in 160 patients with singleton fetuses. Four patients developed preeclampsia, and their banked CVS specimens were matched to 8 control samples from patients with unaffected pregnancies. As part of the routine CVS procedure, if surplus tissue not needed for clinical analyses was available, villi grossly free of decidua and maternal blood were removed from solution in the Petri dish and snap-frozen for microarray analysis.

Tissue Condition Samples
Test chorionic villus first trimester CVS from preeclamptic pregnancy 4
Control chorionic villus first trimester CVS from normal pregnancy 8
Gene Fold Change (log2) Significance Genus Species