To explore chorionic gonadotrophin (CG)-regulated gene expression in the primate corpus luteum (CL) during simulated early pregnancy (SEP), Bishop et al. treated female rhesus monkeys with increasing dosages of hCG mimicking the rise detected during early pregnancy. SEP treatment began on day 9 of the luteal phase. The first day of the luteal phase was defined as the first day of low serum E (<100 pg/ml) following the mid-cycle E surge, with a coincident rise in serum P above 0.2 ng/ml. Individual CL were collected on days 10, 12, 15, and 18 of the luteal phase, representing 1, 3, 6 and 9 days of hCG treatment. Additionally, CL were collected from untreated monkeys on day 10 of the luteal phase to serve as baseline controls.

Tissue Condition Samples
Test corpus luteum day 15 simulated early pregnancy 4
Control corpus luteum day 12 simulated early pregnancy 4
Gene Fold Change (log2) Significance Genus Species