Dunk et al. investigated the differential gene expression profiles of placental microvascular endothelial cells (PlMECs) in pregnancies complicated by severe early-onset intrauterine growth restriction (IUGR) with or without superimposed preeclampsia versus gestational age-matched preterm controls. Severe IUGR preterm placentas were collected from patients with fetal weight ≤10th percentile with AREDV in the umbilical artery and being delivered for obstetrical indications at 24–34 weeks of gestation. Preterm control placentas were collected from preterm pregnancies with appropriately grown fetuses that were delivered following preterm labor at 24–34 weeks of gestation. Preterm placentas with chorioamnionitis were excluded. Placental tissue, taken from the distal (i.e. maternal) side of the placenta, was digested and PlMECs positively selected using tocosylated magnetic Dynabeads labeled with Human Endothelial Antigen lectin.
||intrauterine growth restriction
||gestational age-matched control