Using monolayer cultures of human amnion mesenchymal cells as a model, Li et al. aimed to detail the global programme of gene expression that occurs in response to cytokine challenge. Primary cultures of human amnion mesenchymal cells were prepared from amnion membranes obtained prior to labor at term and challenged with 10 ng/ml interleukin-1β for 1 hour or 8 hours in serum-free media, while control cells were treated with serum-free medium only.

Tissue Condition Samples
Test amnion interleukin-1β challenge for 8 hours 3
Control amnion interleukin-1β challenge for 1 hour 3
Gene Fold Change (log2) Significance Genus Species