Votavova et al. performed a comprehensive analysis of transcriptome alterations induced by smoking in maternal and fetal cells. Samples of peripheral blood, placenta, and cord blood were obtained from pregnant smokers and gravidas without significant exposure to tobacco smoke. Smoking status was confirmed by detection of excessive plasma levels of cotinine. Placental samples were prepared according to the protocol by Sood et al., as described in detail previously.

Tissue Condition Samples
Test umbilical cord blood smoker 19
Control umbilical cord blood non-smoker 45
Gene Fold Change (log2) Significance Genus Species