Santner-Nanan et al. searched for evidence of transplacental programming in cellular immunity by characterizing the gene expression profiles in maternal and fetal regulatory T (Treg) cells. Peripheral blood samples from pregnant women were taken 2-5 hours before delivery, and cord blood was obtained at delivery. Treg cells and non-Treg cells were sorted by Ficoll-Hypaque gradient centrifugation, and total RNA was extracted for microarray analysis.
||non-Treg cells from maternal blood
||non-Treg cells from non-pregnant control