Telugu et al. compared extravillous trophoblast (EVT) cells derived from human embryonic stem cells (hESC) to those obtained from first trimester human placentas in order to evaluate their potential as an in vitro model for investigating EVT invasion. HESC were treated with BMP4 under 4% or 20% oxygen and tested in Matrigel invasion chambers. Both BMP4-treated hESC and primary human placental cells were separated into HLA-G+ (EVT) and HLA-G−/TACSTD2+ (cytotrophoblast) populations with immunomagnetic beads and expression profiles analyzed by microarray.

Tissue Condition Samples
Test cytotrophoblast primary human placental cells (HLA-G−/TACSTD2+) 3
Control cytotrophoblast primary human placental cells (HLA-G+) 3
Gene Fold Change (log2) Significance Genus Species