Hui et al. aimed to determine whether the relative representation of specific organs in term amniotic fluid cell-free fetal RNA would differ from that in second-trimester amniotic fluid. Using global gene expression analysis, the study compared 8 second-trimester amniotic fluid samples from routine amniocenteses to 8 term amniotic fluid samples from women undergoing prelabor cesarean deliveries. Amniotic fluid was collected at cesarean delivery after entry into the uterus and before rupture of the amniotic membranes.

Tissue Condition Samples
Test amniotic fluid term pregnancy 8
Control amniotic fluid second trimester pregnancy 8
Gene Fold Change (log2) Significance Genus Species