To identify genes and pathways correlating with β-glucocerebrosidase (GBA) expression, Jebbink et al. performed microarray expression profiling on placental tissue from normotensive patients with low GBA expression and from preeclamptic patients with high GBA expression. Quantitative RT-PCR was used to determine normotensive and preeclamptic placentas with the most extreme levels of GBA expression prior to microarray analysis. Placental biopsies were obtained immediately after delivery from a macroscopically viable (non-infarcted) central cotyledon from the maternal side.
||preeclamptic + HELLP pregnancy