To investigate paracrine communications that occur at the decidua-trophoblast interface, Hess et al. studied the effects of products secreted from human trophoblasts on global gene expression in in vitro-decidualized human endometrial stromal cells. Human endometrial stromal cells were decidualized with progesterone and treated with conditioned media from either human trophoblasts or non-decidualized stromal cells (control) for 0, 3, and 12 hours.
||trophoblast-conditioned medium for 12 hours
||control medium for 12 hours