To investigate paracrine communications that occur at the decidua-trophoblast interface, Hess et al. studied the effects of products secreted from human trophoblasts on global gene expression in in vitro-decidualized human endometrial stromal cells. Human endometrial stromal cells were decidualized with progesterone and cultured in human trophoblast-conditioned media (TCM), or in conditioned media from non-decidualized stromal cells (CCM) as a control.
||TCM-treated human endometrial stromal cells at 12 hours
||CCM-treated human endometrial stromal cells at 12 hours