To test the hypothesis that expression of genes unique to resident macrophages (Mφs) distinguishes the late remodeled prepartum from unremodeled nonpregnant cervix, Dobyns et al. performed a microarray analysis on perfused cervix, with or without Mφs, from prepartum day 21 postbreeding and nonpregnant rats. Cells from the cervix in the macrophage-depleted groups were subjected to a magnetic bead separation procedure to remove Mφs.

Tissue Condition Samples
Test uterine cervix pregnant cervix with Mφs 3
Control uterine cervix nonpregnant cervix with Mφs 3
Gene Fold Change (log2) Significance Genus Species