To gain more insight into the regulatory mechanisms of trophoblast invasion, Bilban et al. investigated the genome-wide expression profiles of nonmigratory villous cytotrophoblasts and invasive, extravillous trophoblasts. Villous cytotrophoblasts were purified from first trimester placentas and grown in culture for 24 hours. Extravillous trophoblasts were collected from first trimester villous explant cultures seeded on Matrigel after 72 hours.
||first trimester extravillous trophoblasts
||first trimester villous cytotrophoblasts